A Short Course on Investigations in COVID-19

Published 2021-12-01
Platform Udemy
Rating 3.50
Number of Reviews 1
Number of Students 3
Price $19.99
Instructors
Dr P A Shah
Subjects

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This course covers essentials of common investigations used in COVID-19, including RT-PCR, Radiology, CRP & D-dimer.

This course is covered under the following headings:


RTPCR - SAMPLE COLLECTION

1. Introduction

2. Pre-collection

3. Collection

4. Post-collection

5. Transport


RTPCR – RNA EXTRACTION

1. Introduction

2. Components

3. Lysis Buffer

4. Silica Membrane Based RNA Extraction Part 1

5. Silica Membrane Based RNA Extraction Part 2

6. Magnetic Bead Based RNA Extraction Part 1

7. Magnetic Bead Based RNA Extraction Part 2


RTPCR – RNA AMPLIFICATION

1. Introduction

2. What is PCR?

3. Reaction Mixture

4. Step 1 – Denaturation

5. Step 2 – Annealing

6. Step 3 – Extension

7. Thermal Cycles

8. What is RT-PCR?

9. Reaction Mixture


RTPCR RNA QUANTIFICATON

1. Introduction

2. Terminology

3. Spectrofluorometer

4. TaqMan Probe

5. How Does it Work?

6. Amplification Curve

7. Ct Value

8. Sample Report


CHEST X RAY IN COVID 19

1. Resources

2. Imaging recommendations

3. Interpretation of CXR report

4. Probable COVID 19 CXR

5. Non-COVID 19 CXR

6. Baseline CXR in COVID 19


CT CHEST IN COVID 19 PART 1

1. Introduction

2. Ground-Glass Opacities

3. Crazy Paving Pattern

4. Consolidation

5. Reverse Halo Sign

6. Other CT Features

7. Interpretation of CT Report

8. Typical Appearance


CT CHEST IN COVID 19 PART 2

1. Introduction

2. Indications in a suspected case

3. Indications in a confirmed case

4. CO-RADS

5. 25 Points CT Severity Score

6. 40 Points CT Severity Score

7. When not to do a CT Chest


C-Reactive Protein

1. Introduction

2. Values of CRP

3. CRP versus ESR

4. CRP in COVID-19

5. Advantages of CRP

6. High Sensitivity CRP


D-DIMER

1. Introduction

2. D-dimer levels

3. Etiology of pulmonary embolism

4. Risk Factors for Pulmonary Embolism

5. Approach to Pulmonary Embolism

6. D-dimer in COVID-19



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